Journal: STAR Protocols

Article Title: Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

doi: 10.1016/j.xpro.2021.100641

Figure Lengend Snippet:

Article Snippet: Refer to p. 27ff of the Juno System user guide (PN 100–7070 r12) on how to change the interface plate: https://www.fluidigm.com/binaries/content/documents/fluidigm/resources/juno-user-guide-ug-100-7070/juno-user-guide-ug-100-7070/fluidigm%3Afile After pipetting of all the samples, assays and pressure fluid, place the 192.24 IFC into the Juno and run the following script: Load Mix 192.24 GT.

Techniques: Recombinant, Suspension, Saline, Binding Assay, Control, Multiplex Assay, Cell Isolation, Real-time Polymerase Chain Reaction, Software, Gene Expression, Transferring, Fluorescence, Microscopy

Journal: International endodontic journal

Article Title: EGR1 supports the osteogenic differentiation of dental stem cells.

doi: 10.1111/iej.12299

Figure Lengend Snippet: Figure 3 EGR1 depletion indicates a down-regulation of DLX3 and BMP2 (SCAPs and DFCs). (a, b) EGR1 depletion was deter- mined by qRT-PCR analysis after 48 h of transfection with an EGR1-specific siRNA (siRNA EGR1) and nonspecific NS siRNA (NS siRNA) in SCAPs and DFCs. (c, d) A decreased EGR1 expression was also verified at protein level by western blotting. (e, f) The expression of the osteogenic markers ALP, RUNX2, DLX3 and BMP2 was examined after 48 h of transfection by a qRT- PCR. DFCs transfected with the nonspecific siRNA was used for calibration. Grey bars present nonspecific siRNA and black bars EGR1-specific siRNA. (*: Student0s t-test P < 0.05)

Article Snippet: Published by John Wiley & Sons LtdInternational Endodontic Journal, 48, 185–192, 2015186 The early growth response gene 1 (EGR1) expression plasmid pCMV6-Entry was purchased from OriGene Technologies (Rockville, Maryland, USA).

Techniques: Quantitative RT-PCR, Transfection, Expressing, Western Blot

Journal: International endodontic journal

Article Title: EGR1 supports the osteogenic differentiation of dental stem cells.

doi: 10.1111/iej.12299

Figure Lengend Snippet: Figure 2 ODM and BMP2 medium induce EGR1 expression (SCAPs). EGR1 expression in SCAPs was measured after 3 days of osteogenic differentiation using ODM or BMP2 medium and was compared with DMEM standard medium using a qRT-PCR. (*: Student0s t-test P < 0.05).

Article Snippet: Published by John Wiley & Sons LtdInternational Endodontic Journal, 48, 185–192, 2015186 The early growth response gene 1 (EGR1) expression plasmid pCMV6-Entry was purchased from OriGene Technologies (Rockville, Maryland, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: International endodontic journal

Article Title: EGR1 supports the osteogenic differentiation of dental stem cells.

doi: 10.1111/iej.12299

Figure Lengend Snippet: Figure 4 EGR1 over-expression indicates more mineralization after 28 days and shows up-regulation of DLX3 and BMP2 (SCAPs). (a) EGR1 over-expression was determined by qRT-PCR analysis after 48 h of transfection with expression plasmid (pEGR1) and an empty vector (pEV). Western blot analysis confirmed over-expression of the EGR1 protein. (b) Gene expression of the osteogenic markers ALP, RUNX2, DLX3 and BMP2 was investigated after 48 h of transfection using a qRT-PCR. Gene expressions in DFCs after transfection with an empty vector (pEV; grey bars) and with an EGR1 expression plasmid (pEGR1, black bars) were compared. Gene expression after transfection with pEV was used for calibration. (c) Alizarin red staining: grey bars show an empty vector (pEV) and black bars the expression plasmid (pEGR1). (d) Microscopic pictures according to the column chart. (*: Student0s t-test P < 0.05).

Article Snippet: Published by John Wiley & Sons LtdInternational Endodontic Journal, 48, 185–192, 2015186 The early growth response gene 1 (EGR1) expression plasmid pCMV6-Entry was purchased from OriGene Technologies (Rockville, Maryland, USA).

Techniques: Over Expression, Quantitative RT-PCR, Transfection, Expressing, Plasmid Preparation, Western Blot, Gene Expression, Staining